Genomic instability in patients with autosomal-dominant polycystic kidney disease
M Li, S Qin, L Wang, J Zhou - Journal of international …, 2013 - journals.sagepub.com
M Li, S Qin, L Wang, J Zhou
Journal of international medical research, 2013•journals.sagepub.comObjective Autosomal-dominant polycystic kidney disease (ADPKD) is a systemic disorder
affecting multiple organs that results in renal and extrarenal cysts. Patients with ADPKD may
have genomic instability, making them more vulnerable to developing cancer. This study
aimed to investigate latent genomic instability in patients with ADPKD, using single-cell gel
electrophoresis (comet assay). Methods The susceptibility of peripheral blood lymphocytes
to DNA damage induced by X-ray treatment (0.5 Gy) was tested in 20 patients with ADPKD …
affecting multiple organs that results in renal and extrarenal cysts. Patients with ADPKD may
have genomic instability, making them more vulnerable to developing cancer. This study
aimed to investigate latent genomic instability in patients with ADPKD, using single-cell gel
electrophoresis (comet assay). Methods The susceptibility of peripheral blood lymphocytes
to DNA damage induced by X-ray treatment (0.5 Gy) was tested in 20 patients with ADPKD …
Objective
Autosomal-dominant polycystic kidney disease (ADPKD) is a systemic disorder affecting multiple organs that results in renal and extrarenal cysts. Patients with ADPKD may have genomic instability, making them more vulnerable to developing cancer. This study aimed to investigate latent genomic instability in patients with ADPKD, using single-cell gel electrophoresis (comet assay).
Methods
The susceptibility of peripheral blood lymphocytes to DNA damage induced by X-ray treatment (0.5 Gy) was tested in 20 patients with ADPKD using single-cell gel electrophoresis. The percentage of DNA in the comet tail (TDNA%) before and after irradiation was compared between patients with ADPKD and 20 sex- and age-matched healthy control subjects.
Results
Renal and extrarenal cysts were observed in patients with ADPKD. A significantly higher mean TDNA% was determined in patients with ADPKD compared with control subjects (8.85% versus 7.50%). After in vitro irradiation, DNA damage was significantly increased in all participants, but the increase was significantly greater in patients with ADPKD compared with control subjects.
Conclusion
These data suggest that patients with ADPKD have genomic instability, which may trigger renal and extrarenal cyst formation.
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