Chronic granulomatous disease (CGD) is a rare recessive disorder caused by defects in the
NADPH oxidase enzyme complex of phagocytes (neutrophils, eosinophils and monocytes).
CGD phagocytes fail to produce superoxide and other reactive oxygen species following cell
activation (Malech, 1993). The products of oxidase activation can be measured in individual
cells by flow cytometry using specific fluorescent probes that increase fluorescence upon
oxidation (Trinkle et al., 1987). This approach can be used to confirm a diagnosis of CGD …