T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal …
V Brinkmann, CH Heusser - Cellular immunology, 1993 - Elsevier
The development of human functional Ig precursors into plasma cells expressing IgM, IgG,
IgA, or IgE was compared. Purified human B cells were stimulated at limiting dilution with
irradiated EL4 helper cells, IL-2, and IL-4. B cells proliferated exponentially until Day 8 of
culture. Nondividing plasma cells of all isotypes were detectable in ELISPOT assays
between Days 8 and 10 and secreted 1.8±0.7 ng antibody per cell within 24 hr. This
indicates that plasma cells of all isotypes, including IgE, bear a comparable potential to …
IgA, or IgE was compared. Purified human B cells were stimulated at limiting dilution with
irradiated EL4 helper cells, IL-2, and IL-4. B cells proliferated exponentially until Day 8 of
culture. Nondividing plasma cells of all isotypes were detectable in ELISPOT assays
between Days 8 and 10 and secreted 1.8±0.7 ng antibody per cell within 24 hr. This
indicates that plasma cells of all isotypes, including IgE, bear a comparable potential to …
